PURPOSE: We hypothesized that the concurrent training [high-intensity interval training (4×4 min intervals at 85-95% maximum heart rate [HRmax], interspersed with 4 min of recovery at 75-85% HRmax) + progressive resistance training (12-15 repetitions per set, at 50-70% of one repetition maximum with 60s of recovery)] induces the highest metabolic perturbations and therefore the highest hormonal responses compared to the progressive resistance training and the high-intensity interval training protocol in a cohort of Latin-American overweight adults (age 18-30 years old). METHODS: Randomized, parallel-group clinical trial among fifty-one men (23.6±3.5 yr; 83.5±7.8 kg; 28.0±1.9 kg/m2), physical inactivity (i.e. <150 min of moderate-intensity exercise per week for greater than 6 months), with abdominal obesity (waist circumference ≥ 90 cm) or body mass index ≥ 25 and ≤ 30 kg/m2 were randomized to the following 4 groups: high-intensity training (n=14), progressive resistance training (n=12), concurrent training [high-intensity and progressive resistance training (n=13)], or non-exercising control (n=12). Total- and free-testosterone and total-testosterone/cortisol-ratio assessments (all in serum) were determined before (pre) and 1-min post-exercise for each protocol session. RESULTS: Decreases in cortisol and total-testosterone/cortisol-ratio levels were observed; −57.08 (95%CI, −36.28 to −77.88; d=2.06) and −0.021 (95%CI, −0.012 to −0.032; d=1.49), respectively in the high-intensity training group. In per-protocol analyses, the combined training group had greater changes in cortisol levels (−54.49, 95%CI, −15.28 to −93.69; d=1.33) and total-testosterone/cortisol-ratio (−0.017, 95%CI, −0.004 to −0.030; d=0.90) vs the high-intensity training group, but not the other interventions. Analyses of covariance revealed no significant change in the total- and free-testosterone between groups over time. CONCLUSIONS: The present data indicate a concurrent, or high-intensity training reduced cortisol and total-testosterone/cortisol-ratio levels significantly in physical inactive adults. Further study is required to determine the biological importance of these changes in hormonal responses in overweight men. TRIAL REGISTRATION: ClinicalTrials.gov NCT02915913.