BACKGROUND: Self-perception and Lifestyle have a significant influence on athletes´ performance. Research in Rugby had been focused mainly on the assessment of physiological and anthropometrical player´s profiles as well as injuries incidence. PURPOSE: The aim of this study was to evaluate the self-perception of Fitness and Lifestyle in Rugby players in Colombia METHODS: A cross-sectional study was conducted with Rugby players from 37 cities in Colombia aged 18 years and older, with at least one year of sport practice. Fitness self-perception was assessed with the International Fitness Scale (IFIS) and Lifestyle was evaluated with the FANTASTIC Questionnaire. Statistical analysis was performed with central tendency measures, standard deviation, t- student and chi-square. RESULTS: 479 players were surveyed. 68% were men, 53.7% played at Backs position and 46.3% as Forwards position. The age average was 24.6 (±4.4) years old and a practice experience of 4.2 (±3.8) years. Most of the participants classified their Fitness and Its components: Cardiorespiratory endurence, Strength and Speed /Agility as “Good” (58%, 47%, 53%, 44% respectively), while Flexibility was categorized between “Acceptable” 36% and “Good” 34% mainly. Significant differences were found by position on the field (Backs and Forwards) in the Self-perception of the Strength in players (p = 0.000). Regarding to Lifestyle, evaluated with the FANTASTIC Questionnaire, the average of overall score was 75.3 (±10.2). According to this, the majority of the players, (54.7%, n = 262), reported their lifestyle in the category “Good”. Significant differences were also found by position on the field in the total scores (p = 0.004). It highlight the difference between Forwards and Backs in the category “Aggressiveness” belonging to Personality Type domain (p = 0.04). CONCLUSION: Our results are consistent with the pilot study conducted previously on the same population. The players show a good self-perception of their Fitness and its components as well as a good Lifestyle. The differences found, might be explained according to the characteristics of the player’s position on the field. Furthermore studies are necessary.