Objective: To determine whether there is an association between Multiple Sclerosis (MS) and vitamin D (VitD), Epstein-Barr virus (EBV) antibodies or lifestyle. Background: It is known that environmental and genetic factors are implicated in the pathogenesis of MS. Currently there are no studies evaluating the correlation between VitD, EBV antibodies or lifestyle in patients with MS near the equator. Design/Methods: With a case-control study we applied a questionnaire which included dietary habits, sunlight exposure, physical activity, medical and family history, and hormonal factors to 87 patients with MS and 87 controls. A blood sample was collected to measure VitD levels and EBV antibodies. A univariate analysis using logistic regression was performed with MS status as the outcome and the following independent variables. Results: 87 patients were analyzed, 65.5% were females and 50% of them hadMS. Mean age in men and women was 41.4 and 49.16 years, respectively. 87.95% were RRMS, 7.32% SPMS and 4.82% PPMS. MS patients showed significantly higher levels of VitD compared to controls (31.3±22.2 vs. 25.6±9.6; p=0.047). EBV nuclear antigen (EBV-NA) antibodies were elevated in patients with MS (OR=4.32, CI95% 1.3–13.6, p=0.012). Univariate regression showed significant association between MS and alcohol (OR=11.4, CI95% 1.38–95.57, p=0.024) and soft drinks (OR=2.8, CI95% 1.2–6.3, p=0.013) consumption during adolescence, and no association with a gluten free diet (OR=1.01, CI95% 0.19–5.15, p=0.989). Conclusions: Vitamin D levels were higher in the MS patients when compared with controls, although it should be taken into account that patients were on vitamin D supplementation. Alcohol and soft drink consumption during adolescence along with EBV-NA seropositivity were associated with higher risk of MS. There was no association with a gluten free diet. Additional dietary information will be available at the presentation. Study Supported by: Hospital Universitario Fundacion Santa Fe de Bogota. Universidad El Bosque. Universidad de los Andes. Disclosure: Dr. Toro has received personal compensation in an editorial capacity for New England Journal of Medicine Journal Watch Neurology and Multiple Sclerosis and Related Disorders. Dr. Cortes has nothing to disclose. Dr. Diaz-Cruz has nothing to disclose. Dr. Reyes has nothing to disclose. Dr. Reyes-Mantilla has nothing to disclose. Dr. Duque Ramirez has nothing to disclose. Dr. Patino has nothing to disclose. Dr. Torres has nothing to disclose. Dr. Rios has nothing to disclose. Dr. Cuellar Giraldo has nothing to disclose.