Introduction: Hearing loss is one of the most frequent sensory alterations, affecting approximately one in 500 births, and it may occur de novo at any age in healthy individuals. Thanks to massive sequencing techniques, such as whole exome sequencing, and the use of strict variant filtering protocols, more than 100 loci related to non-syndromic hearing loss have been identified. The aim of this study is to design a protocol for the identification of low coverage nucleotide sequences from exome sequencing data, particularly in the genes responsible for non-syndromic autosomal dominant hearing loss.