Because the genus Puya is one of the most important food resource for Andean bears, the nutritional content of its leaves might be important for choosing the individuals, species or quantity in order to meet the bears’ nutritional requirements. The objective of this research was to compare whether or not there are differences between mature and young leaves of two Puya species ( Puya goudotiana and Puya santosii ) in the paramo ecosystems of the Guavio Region, Colombia. Two leaves per individual were collected from the base of the leaf. Soil samples were also collected in each sample point in order to evaluate if soil related to leaf nutrient content. Results showed marked differences between species. P. goudotiana exhibited the highest concentrations of all elements except for calcium. Also, significant differences were found between conspecific mature and young leaves. The latter showed higher values of phosphorus, potassium and nitrogen, meanwhile mature leaves were higher in calcium. No relationship between soils chemical composition and leaf nutrient content was found. The reasons for these differences and the consequences in the Andean bear diet are discussed.