A sensitive Northern hybridization method, developed to detect viroids in commercial species and cultivars, was used to begin surveys for citrus viroids in Colombian orchards.Analysis of seven samples collected in the Tolima Region showed that five Tahiti lime sources were infected with HSVd and CVd-III, whereas two Mexican lime sources were viroid-free.Three additional Mexican lime sources collected in Magdalena municipality were also viroid-free, whereas two Tahiti lime sources were infected with HSVd and CVd-III or CEVd, HSVd and CVd-III.Cultivars of Valencia sweet orange, Mexican lime and Tahiti lime, maintained in the germplasm collection of Palmira, were all viroid-free.Of the three citron sources, also maintained in the germplasm collection, two were viroid-free and one was infected with CEVd and CVd-III.The base sequences of the viroids from the infected sources were determined and compared with the reference sequences.This is the first report on citrus viroids in Colombia.