Based on results of different works carried out under the project of hydrogen and sustainable energies of the Department of Energy and the perspective shown by Bergues et al [6], concerning the presentation and interpretation of results obtained by Aleman [21], Sandoval and Yanez [10] and Morales and Argueta [22]; it is established a rigorous method for the analysis of the results obtained using as a model the Gompertz equation, and its first and second derivatives as indicators, that focuses on dark fermentation bio-hydrogen production, from the perspective of the conversion of the product –i.e., not of reagents–, for purposes of its maximization and operation control.