<p><strong>Abstract</strong></p><p><strong>Introduction:</strong> Acute Respiratory Infection (ARI) is a heterogeneous group of viral and bacterial respiratory pathologies including <em>Chlamydophila pneumoniae</em> (CP) and <em>Mycoplasma pneumoniae</em> (MP) that are not routinely identified; these infections in the older adults have mortality rates 3 to 5 times higher than that recorded in other age groups. <strong>Methods:</strong> this study was conducted prospectively to determine the proportion of atypical bacterial pathogens in older adults with ARI in Bogotá. Microbiological diagnosis was determined by real-time PCR (qPCR) in samples of respiratory origin and serology for antibodies IgG, IgA and IgM to MP and CP. <strong>Results:</strong> A total of 71 patients were enrolled from 2012 to 2013. Upper respiratory infections were diagnosed in the 69% of patients and lower respiratory infections in 31%. MP was identified in 9.8% and CP in 8.5%. <strong>Conclusions:</strong> these findings indicated that CP and MP must be viewed as a significant etiological agent of ARI in older adults in Bogotá.</p>