Among the eight species of the genus Sphoeroides (family Tetraodontidae) that inhabit the Tropical Eastern Pacific only Sphoeroides lobatus has been reported in more than onegroup of oceanic islands: Galápagos, Revillagigedo and Isla del Coco. This work presents the first documented record of the longnose puffer (S. lobatus) at Malpelo Island, Colombian Pacific.