The optimal strategy for controlling Multidrug-resistant organisms (MDRO) spread and infection remains debated. Screening is useful in high-risk units to identify the reservoir and to initiate contact precautions. Screening (S) and Decolonization (Dc) may decrease the infection risk in colonized patients. The objective of this study was to determine the risk of developing MDRO infections in patients colonized by CRE (Carbapenem-resistant Enterobacteriaceae) and MRSA (Methicillin-resistant Staphylococcus aureus) in a tertiary care hospital with a CS/Dc program (Chlorhexidine bathing) in Cali - Colombia. S/Dc program was performed between April 2014 and April 2016 in adult patients who were referred or readmitted after being hospitalized for ≥ 8 days. Screening was performed with nasal and rectal swab (ChromID™ MRSA agar and ChromID™ CARBA agar). CHXD bathing was applied in all the patients of the S/Dc program. 426 patients were screened,; 47% were colonized by at least one of the following MDRO: CRE (71%) and MRSA (29%). We found a non-significant trend of lower rate of infections due to CRE in prior CRE colonized patients. (RR 0.75 (95% CI: 0.5–1.0), P = 0.057). No increase in MRSA infection risk was founded in MRSA colonized patients (RR 0.92 (95% CI: 0.6–1.4), P = 0.68). Screening and CHXD bathing in patients colonized with CRE/MRSA could reduce MDRO infection, or at least attenuated infection risk in these colonized patients. All authors: No reported disclosures.