Li-ion batteries currently are the most used power source of different electric and electronics systems. Several works study the temperature effect on rechargeable batteries’ performance, but there are limited experimental results of non-rechargeable batteries. This paper presents the thermal effects on non-rechargeable Li-ion battery CR2 model, exposing them to different temperatures to compare it with the manufacturer's data. The result of this research shows that thermal shock and extreme temperature decrease discharge time or operation voltage which impact their performance.