The genus HolotheleKarsch, 1879 has a confusing taxonomic history, mainly due to a imprecise and outdated generic diagnosis, perpetuated since its original description. In this work, we propose a new diagnosis for the genus, redescribe the type species Holothele rectaKarsch, 1879 and propose a few taxonomic changes. Holothele longipes is here considered as a senior synonym of Holothele rectaKarsch, 1879, Stichoplastus sanguiniceps F. O. P.-Cambridge, 1898, Dryptopelmides rondoniLucas & Bücherl, 1972, Dryptopelmides ludwigiStrand, 1907. We also provide updated geographic distribution records for Holothele longipes and propose the revalidation of ScopelobatesSimon, 1903.