The degenerative myelopathy in dogs is caused by SOD1 mutation c.118G>A, described previously. The penetrance of this genetic disease is not well understood, considering animals can express symptoms being carriers. In Colombia, its incidence and risk for pure breed German shepherd dogs is unknown. We extracted DNA from sampled hair roots and buccal swabs of 36 breeding animals of the Colombian German Shepherd Dogs Association (APPA). We did a monoplex PCR and sequencing to determinate the mutation in the SOD1 gene. We found seven carriers in 36 animals, with an allele frequency of 0.097. We estimate that the entire population frequency can be about 0.1, which is higher than what is reported in other German Shepherd populations. It is the first time that this genetic test was used in Colombia, so the implementation of it can be useful to take down the prevalence of this disease, to avoid breeding between carriers.