The following article is intended to inform the assessment or trend in attitudes toward science and attitudes towards learning Biology involving the sixth, seven, ninth and eleventh grade in Justo Víctor Charry school.This research used qualitative design technique supported by interviews with scale item; internal validation was done with Cronbach's alpha.To obtain the trend in attitudes Likert scale was used and the construction of assessment criteria relevant to school entailed results to conclude that the rate of development of attitudes toward science and learning attitudes decreases with increasing the educational level, and they did the categories responsibilityautonomy and problem solving in learning attitudes toward Biology directly affect 1