The phyllosphere supports a large and complex bacterial community that varies both across plant species and geographical locations. Phyllosphere bacteria can have important effects on plant health. The sweet chestnut (<em>Castanea sativa</em> Mill.) is an economically important tree species affected worldwide by the fungal pathogens <em>Cryphonectria parasitica</em> and <em>Phytophthora cinnamomi</em>. We examined the culturable phyllosphere bacterial community of the sweet chestnut at two nearby locations in Central Spain in order to know its geographical variability and to explore its potential as source of biological control agents against these two pathogenic fungi. The bacterial diversity at strain level was high but it varied significantly between locations; however, phylotype richness and diversity were more comparable. The isolates were affiliated with the phyla <em>Actinobacteria</em>, <em>Firmicutes</em> and <em>Proteobacteria</em>. Most of them were members of recognized bacterial species, with a notable proportion of representative of the genera <em>Dietzia</em> and <em>Lonsdalea</em>, but a small fraction of the strains revealed the existence of several potential novel species or even genera. Antagonism tests showed the occurrence in the chestnut phyllosphere of bacterial strains potentially useful as biological control agents against the two pathogenic fungi, some of which belong to species never before described as fungal antagonists. Chestnut phyllosphere, therefore, contains a great diversity of culturable bacteria and may represent an untapped source of potential biocontrol agents against the fungi causing blight and ink diseases of this tree species.