Tissue Engineering (TE) is a multidisciplinary area that involves many branches of knowledge such as biology, medicine, bioengineering, chemistry, etc. The TE aims to restore the organ or tissue with reduced functionality due to illness, accident or other external factors. The TE overcomes the limitations of the medicine related with improving quality of life in patients. The goal of the book Tissue Engineering and Applications is to show to the expert and inexperienced reader, an analytical perspective of the treatments used for a wide range of diseases, based on well supported evidence in therapeutic techniques and applications of TE reported in scientific literature. This book delves into concepts such as scaffolding, decellularized matrix, biomaterials and implants, as well as the biological support of the actuation mechanisms in cardiovascular, neural, skin and bone tissues.