Much of the diversity of tomato is found in wild forms, the most important being the Solanum lycopersicum L. var. cerasiforme and S. pimpinellifolium. The objective of this research was to assess the genetic diversity of 30 introductions of cherry tomato with 36 microsatellite molecular markers. The study was conducted at the Plant Transformation Research Center (PTRC) of the University of California. A dendrogram was built using the Dice-Nei and Li similarity index and the UPGMA clustering method, where introductions were differentiated without preserving a distribution pattern obeying the geographical area of origin. A coefficient of genetic differentiation was found (Fst = 0.3474), showing a high genetic differentiation of the introductions; those from Brazil, Ecuador, and Peru were the most genetically diverse, presenting 100% of polymorphic loci. The molecular variance analysis indicated a variation of 11% between the groups and 89% within the same. The broad genotypic variability of the evaluated introductions favors the possibility of selecting those for genetic improvement and sustainable use of the species.