ABSTRACT This survey presents the first species list of the poroid fungi (Polyporales and related genera) from Parque Estadual da Serra do Mar, Núcleo Santa Virgínia, the largest area of the Atlantic forest in Brazil. A total of 68 species, 38 genera and ten families were found in the studied area. Antrodiella luteocontexta, Ceriporiopsis flavilutea, Diplomitoporus navisporus, Flaviporus venustus, Grammothele fuligo, Oxyporus latemarginatus, Perenniporia cremeopora, Postia subcaesia and Postia tephroleuca are recorded for the first time to São Paulo State and Dichomitus campestris and Postia undosa represent the first records in Brazil. Full description of the new records in Brazil, comments about the new records in São Paulo State, as well as pictures and an identification key are provided.