The Mesembrinellidae (Diptera: Oestroidea) are a group of exclusively Neotropical flies, with 38 recognized species distributed in nine genera. A total of 11 of these species, present in seven genera, were known to occur in Colombia. Huascaromusca obscura Wolff, sp. nov. and H. cordillera Wolff & Ramos-Pastrana, sp. nov. are herein described and their male terminalia are illustrated. Based on a prior molecular study of the family, Giovanella carvalhoi Wolff et al., 2013 is transferred to Huascaromusca Townsend. A key to all known species of Huascaromusca is provided.