Due to its outstanding properties, the titanium and its alloys have been widely used in the dental and orthopaedic fields as biomaterials. The TiO2 nanotubes surface and the texturized process by laser engraving enables significantly accelerated osteoblast adhesion on the biomaterial. For this reason in this paper, the HOS cell responses on TiO2 nanotubes fabricated on Ti6Al4V alloy and texturized by laser engraving were evaluated. The test surfaces were carried out on smooth Ti6Al4V as control, TiO2 nanotubes (NT) and surfaces with micropoints obtained by laser engraving, with 1mm spacing (NTP1) and 0.5mm (NTP2). The results show that the texturized process enables decreases the contact angle thus improving wettability of the TiO2 nanotubes surface. The NTP1 and NTP2 surfaces show excellent cell adhesion and spreading on the surface, which is evident in epifluorescence microscopy images. Furthermore, the NTP1 and NTP2 surfaces improved the cell proliferation at 18% and 16% respectively in relation with NT surface, showing that the laser texturing improves cell response of TiO2 nanotubes.