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Color separation of H&E stained samples by linearly projecting the RGB representation onto a custom discriminant surface

Acceso Cerrado
ID Minciencias: ART-0000305065-431
Ranking: ART-GC_ART

Abstract:

This paper presents a novel color separation method for Hematoxylin-Eosin (H&amp;E) stained Histopathology Images. The whole (<i>R;G;B</i>) space of the input image is projected to a family of surfaces connecting the distributions of a series of [<i>(R + B)<sup>2</sup>=B;G</i>] planes that divide the cloud of H&amp;E tones. Such projection is then used to cluster both the Hematoxylin and Eosin samples, from where the color basis are then derived. The projection presented herein is more resilient to noise and therefore the separation process is less hampered when the staining properties largely vary. Unlike other normalization methods evaluated by comparing with gold standard images, the power of the method was demonstrated by thresholding the resulting Hematoxylin image with the well-known Otsu's method and the number of detected nuclei was compared with two manually annotated datasets. Despite the simplicity of this approach, the detection sensitivity was 71 % and 67 %, respectively

Tópico:

AI in cancer detection

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Información de la Fuente:

SCImago Journal & Country Rank
FuenteProceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Cuartil año de publicaciónNo disponible
Volumen10160
IssueNo disponible
Páginas101600P - 101600P
pISSNNo disponible
ISSN0277-786X

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