Corn is a product very susceptible to fungal contamination often facilitated by the grain storage conditions. Thus, the sanitary quality of seeds it may be compromised due to the attack of pathogens that interfere with their viability. The study aimed to identify, quantify and evaluate the occurrence of fungi, and check the incidence of mycotoxins (aflatoxin and zearalenone) in corn grain in post harvest. 7 were collected samples of corn grain in properties in the city of Jataí / GO region. The experiment was developed in the laboratory of Goiás State University, Campus of Jataí-GO. The experimental design was a randomized block for 7 treatments and five repetitions. Step A of the research was the identification of different fungal species, macroscopic and microscopic characteristics were analyzed. The B phase of the project was the mitoxinas detection in fungi found. In all samples the fungus were identified, Aspergillus flavus, Fusarium moniliforme, Fusarium roseum, e Penicillium sp. The presence of aflatoxigênico fungus and the production of aflatoxins were found in some samples of corn kernels that had contamination levels above the official limit (20 mg / kg) permitted by the Ministry of Health / ANVISA and MAPA.Para citar este artículoReges, J., Nogueira de Jesus, N., Duarte, S., Henrique de Souza, M., Santos, I., Moreira do Santos, S. & Walleria, J. (2016). Ocorrência de fungos e micotoxinas em grãos de milho em Jataí-GO, Brasil. Rev. Colomb. Investig. Agroindustriales, 3(1), 34-39. DOI: http://dx.doi.org/10.23850/24220582.342