An inexpensive double optical tweezers has been used for trapping and manipulating microorganisms and micro-sized crystals with regular and irregular shapes.The intensity and spatial location of the traps were controlled through mirror displacements devices.A stable infrared laser beam with tunable power was used.The particles were manipulated and observed in an inverted microscope adjusted to locate the infrared trapping laser beam into the holder.A bacilliform bacterium with an approximate size of 12.0µm was captured by its two elongated ends, exerting control of their displacements and rotations.Also, regular micro-crystals measuring approximately 10.0µmx4.0µmx0.5µmwere captured, achieving nanometric control of their displacements and rotations in two dimensions, parallel to one of their faces.