With the aim of isolating and inoculating diazotrophics bacteria, which is an efficient biological nitrogen fixation (BNF) in rice in Vitoria da Conquista - Ba, the present study was carried out in three steps. In the experiment of inoculation of diazotrophics bacteria, BRS Tropical and BRS MG Curinga, inoculation with the strain of bacterium ZAE 94, absence of inoculation and four doses of N (0, 20, 60 and 100 kg ha-1) were used. The control plants were used for the implementation of the isolation of bacteria efficient as the BNF. Final experiment was carried out with the three isolated strain, the ZAE 94, the absence of inoculation and the interaction with doses of N. Inoculation with strains of diazotrophic bacteria, guaranteed an increase in agronomic variables studied with positive effects on production and grain up to 208.5%.   Key words: Biological nitrogen fixation (BNF), Herbaspirillum seropedicae, grain production.