This article raises the question of whether recently implemented legislation in Colombia and Brazil (1) provides the necessary tools to prevent the harms of wildlife trafficking (WLT) and (2) influences humans’ practices concerning the use of nonhuman animals. These questions are investigated from the dual perspectives of green criminology and public policy. The analysis is based on a qualitative empirical study undertaken in Colombia and Brazil whereby we discuss the function of the legislation in Colombia and Brazil in preventing illegal WLT. We consider the legitimacy of different practices of WLT and evaluate them with respect to species justice and environmental justice.