Phenolic lipids are a series of naturally-occurring compounds which are produced, among others, by species of the family Poaceae, such as barley (Hordeum vulgare L.). Such compounds have been described with significant antimicrobial properties. Therefore, as part of a program for the search for antifungal compounds, an acetone extract and depurated fractions were obtained from grain flour of barley (andina variety) and tested in-vitro against Furarium oxysporum. The acetone-soluble extract showed high alkylrecorcinols content compared to previous works, whose chromatographic profile led to identify at least eight major alkylresorcinols. The purification of the extract (monitored with specific revealer) led to obtain three alkylrecorcinol-enriched fractions. Both the crude extract and the purified fractions showed antifungal activity at different levels, with a dose-dependent response. The treatments showed ED50 values between 3.3-24 μg mL-1. However, enriched fractions showed greater activity to that of crude extract, indicating that the direct effect of alkylresorcinols on the phytopathogen is representative to inhibit their growth. All of the above-mentioned facts suggest these compounds as good candidates for controlling this phytopathogen.