This paper presents the design of a supervisory system of an Anaerobic Biodigestor pilot plant located at Universidad de Ibagué in Colombia to produce Biogas. This contribution includes the design of a reliable low-cost instrumentation; whereby, the correct selection, implementation, and calibration of sensors of temperature, pH, CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> , CH <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> , and gas flow play an important role in accomplishing a good design. The supervisory system is used to perform tasks of fault-detection and on-line measuring; where the last one is essential for modeling by extracting kinetic information of the degradation process and automatic control. Labview <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> was chosen as the software platform to develop the supervisory system.