Mesovelia tuberculata sp. nov. is described and illustrated based on material from the Pacific Region of Colombia. It can be recognized by the following characters of the male: mesofemur without a row of spinules; posterior margin of abdominal tergum VIII with a weak notch; abdominal pleuron VIII with an anterodorsal tubercle; abdominal sternum VI with the posteromedian projection rounded and fringed with black spinules; and paramere with margins converging and apex single. In addition, we present data concerning M. zeteki Harris & Drake and M. mulsanti White, and an identification key to species of Mesoveliidae from Colombia.