Dengue is an infectious disease with a great impact in tropical and sub-tropical regions, particularly at the Quindio (Colombia). This disease generates a cost overrun in the health care institutions. We propose the implementation of a mathematical tool for the entomological and epidemiological analysis of the Dengue. A simulation model accounting for three random endemic communities has been analyzed. Prophylactic and chemical controls of the mosquito population are used. Finally, it is treated the time evolution of the basic reproduction number using the EpiEstim book of the statistical R software. We have used epidemiological data of Armenia-Quindio from 2012 to 2013.