Background & Objectives: Anoxia produces energy uncoupling of mitochondria, depleting adenosine tri-phosphate and homeostasis changes of hydrogen, sodium and calcium. These events activate proteolytic enzymes and impair the ability of cell volume regulation in both endothelial cells and macrophages. This phenomenon, coupled with the change of the production ratio of NO / ET-1 contributes to narrowing of the capillaries and microcirculatory dysfunction. The restoration of blood flow worsens the injury. Reperfusion stimulates the endothelial expression of adhesion molecules that interact with PMN and platelets, allowing the subendothelial infiltration of the first and entrapment in the microvasculature which is favored by vasoconstriction, endothelial swelling and decreased flexibility of the cell membrane. OBJECTIVE: To determine whether hypertonic saline 7.5% (2400 mosm) decreases ischemia / reperfusion injury in liver, kidney and ileum. Materials & Methods: Randomized experimental study in pigs, with suppression of blood flow through the aortic clamping above the celiac artery for 15 minutes and reperfusion for 60 minutes. Ten minutes before aortic clamping, saline hypertonic 7,5% was infused to experimental group, and normal saline 0,9% was infused to control group. Hemodynamic parameters, blood gas analysis, liver and kidney function, and histopathological strains for TNF-alpha and ET-1, were carried out. Results: After reperfusion, the group treated with hypertonic saline exhibited a systolic index increased to baseline, while the systolic index in the control group showed a significant drop when compared to baseline. There were no significant differences in tissue expression of TNF-alpha and IL-10. Conclusion: The use of hypertonic saline before the ischemic insult improves ventricular function after reperfusion and reduces renal dysfunction. References: 1. Adams JA. Endothelium and cardiopulmonary resuscitation. Crit Care Med 2006 Dec;34(12 Suppl):S458-65. 2. Attuwaybi B, Kozar RA, Gates KS, Moore-Olufemi S, Sato N, Weisbrodt NW, et al. Hypertonic saline prevents inflammation, injury, and impaired intestinal transit after gut ischemia/reperfusion by inducing heme oxygenase 1 enzyme. J Trauma 2004 Apr;56(4):749-58; discussion 758-9. 3. Bracht H, Takala J, Tenhunen JJ, Brander L, Knuesel R, Merasto-Minkkinen M, et al. Hepatosplanchnic blood flow control and oxygen extraction are modified by the underlying mechanism of impaired perfusion. Crit Care Med 2005 Mar;33(3):645-653. Disclosure of Interest: None declared