The aim of this study was to evaluate the taxonomic and functional diversity of corticolous lichens under the influence of different micro-climatic conditions in the Botanical Garden of Popayán. We considered 11 contrasting stations distributed in the area, selecting five individuals of Cecropia angustifolia per station. We worked with quantitative sampling locating a quadrant of 0.50 ξ 0.20 m2 on the trunk of each tree, where the percentage of cover for each species was estimated. Microenvironmental parameters, such as light, temperature and relative humidity, CAP and the pH of the bark were evaluated. A total of 63 lichen species was recorded. The results showed that light and temperature had positive correlation with species richness and cover of fruticose and foliose lichens, while relative humidity was negatively related to richness and positively with crustose cover. Lichen distribution at the Popayan Botanical Garden is mainly affected by light, temperature and relative humidity