Nowadays the transportation systems show new challenges in management and supervision issues due to amount of vehicles and the variability and extension of the roads. New technologies have been developed to get information about traffic in cities. Nevertheless, new frameworks with different technologies applied to traffic monitoring have not been well defined. In this paper, a new framework with Radio Frequency Identification technology (RFID) applied to road traffic monitoring is exposed. In order to validate the designed framework, an experiment in a university campus was carried out, concluding the effectiveness of the RFID technology for traffic monitoring.