Introduction. During the last years appearance of wild animals in places such as zoos and ecological parks has become common. Introduction of wild species in a rehabilitation process should include a microbiological evaluation, which leads control of microbial species that may pose a factor of anthropozoonotic risk for human beings serving, or visiting such reservoir places, and care of exotic and wild fauna. Objective. The purpose of the study is to establish frequency of intestinal parasites in exotic and wild fauna at Santa Fe Zoo, in Medellin, Colombia. Methodology. An Ambi-spective descriptive research was performed at the veterinary laboratory of Santa Fe Zoological Park. The evaluated term include 2007 through 2010. Results. During the four-year period 1,089 samples of faecal matter were processed, corresponding to mammalian animals, (70,8%); general positive incidence was 30,5%; the highest percentage was found in the mammalian class with 76.5%, affecting more than 30 species of animals; positive trend in the various Classes of animals was similar during the tested years.