PURPOSE:The purpose of this study was to examine the physical activity (PA) levels and perceived benefits and barriers to PA on a satelite campus (Ciudadela Robledo) of the University of Antioquia, in Medellin, Colombia. METHODS:A random sample of 209 participants was selected to participate in this study [students (N=92), faculty (N=72) and staff (N=45)]. The Global Physical Activity Questionnaire (GPAQ) was used to estimate levels of physical activity (PA) and the Exercise Benefits / Barriers Scale (EBBS) was used to examine perceived benefits and barriers of physical activity. The surveys were collected during the second half of 2012. Chi-square was used to compare differences between gender and university affiliation (i.e. students, faculty, or staff). RESULTS:In this random sample, males reported higher proportions of high-intensity PA compared to women (56% vs. 44%; P< 0.05). However, we did not find significant differences between the three affiliation groups in regards to levels of PA (P = 0.321). Nonetheless, students reported higher levels of high-intensity PA compared to faculty and staff (51%, 49%, 47%, respectively). In addition, no significant differences were observed for the perception of benefits and barriers to PA between males and females (P=0.624). However, faculty members reported greater perceived benefits and greater barriers to PA compared to students and staff members (P=0.004). CONCLUSIONS:The results of this study provide insight into the creation of strategies to improve PA programs within the university and minimize barriers to PA, while increasing adherence to these programs.