Three new and four known species of earthworms (Oligochaeta: Glossoscolecidae) from the department of Caquetá inColombia’s Amazon region were studied. Species belong to the following three families: Glossoscolecidae: Andiodrilusnonuya sp. nov., Andiorrhinus (Turedrilus) yukuna sp. nov., Pontoscolex (Pontoscolex) bora sp. nov., and Pontoscolexcorethrurus (Müller, 1857); Acanthodrilidae: Dichogaster (Diplothecodrilus) affinis (Michaelsen, 1890), Dichogaster(Diplothecodrilus) bolaui (Michaelsen, 1891), and Dichogaster (Diplothecodrilus) saliens (Beddard, 1893); andOcnerodrilidae: Ocnerodrilus occidentalis Eisen, 1878. With these new records, the earthworm fauna of Colombia nowcontains 139 species. Keys to differentiate species of Andiodrilus Michaelsen, 1900, Andiorrhinus (Turedrilus) Righi, 1993, and Pontoscolex (Pontoscolex) (Müller, 1857) are provided.