Weaning is the most critical phase of the pig, causing intestinal disorders and diarrhea. To prevent this, have been used antibiotic growth promoters (AGP), generating medicine residues in the final product. Alternatively, bacteria that promote protein synthesis and lipid metabolism have been used probiotic. The aim was to identify changes in plasma metabolites in weaning pigs fed different probiotic strains. 80 piglets (weaned at 21 days) consumed two diets: Commercial diet with and without the addition of antibiotic (with added probiotic L. casei, L. acidophilus or E. faecium) in water. Blood samples were taken (15, 30 and 45 post-weaning) to determine plasma metabolite changes. An increase (P<0,05) in plasma levels of calcium, phosphorus, alkaline phosphatase and glucose was observed; also decreased levels of creatinine, triglycerides and ALT (alanine aminotransferase) in animals fed E. faecium, compared with those who consumed antibiotic. Probiotics (particularly E. faecium), can be considered as an alternative to the use of AGP in pig diets, by improving the state of digestive organs and act as natural growth promoters because they improve the metabolic state of the animal with positive changes in blood levels of calcium, phosphorus and glucose.