Two new species of Endophragmiella are described and illustrated. Endophragmiella bicolorata is characterized by (1–)2(–3)-septate conidia with a dome-shaped hyaline to subhyaline apical cell and brown basal and middle cells, a pigmentation pattern not previously described for species of this genus. Endophragmiella cantabrica is distinguished from species with 1-septate conidia by oblong, ellipsoidal, or sometimes ovoid conidia comprising two equal and uniformly pale brown cells.