Abstract This article documents the addition of 123 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brenthis ino , Cichla orinocensis , Cichla temensis , Epinephelus striatus , Gobio gobio , Liocarcinus depurator , Macrolophus pygmaeus , Monilinia vaccinii‐corymbosi , Pelochelys cantorii , Philotrypesis josephi , Romanogobio vladykovi , Takydromus luyeanus and Takydromus viridipunctatus . These loci were cross‐tested on the following species: Cichla intermedia , Cichla ocellaris , Cichla pinima , Epinephelus acanthistius , Gobio carpathicus , Gobio obtusirostris , Gobio sp. 1 , Gobio volgensis , Macrolophus costalis , Macrolophus melanotoma , Macrolophus pygmaeus , Romanogobio albipinnatus , Romanogobio banaticus , Romanogobio belingi , Romanogobio kesslerii , Romanogobio parvus , Romanogobio pentatrichus , Romanogobio uranoscopus , Takydromus formosanus , Takydromus hsuehshanesis and Takydromus stejnegeri .