Abstract This article documents the addition of 238 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alytes dickhilleni , Arapaima gigas , Austropotamobius italicus , Blumeria graminis f. sp. tritici , Cobitis lutheri , Dendroctonus ponderosae , Glossina morsitans morsitans , Haplophilus subterraneus , Kirengeshoma palmata , Lysimachia japonica , Macrolophus pygmaeus , Microtus cabrerae , Mytilus galloprovinciali s, Pallisentis ( Neosentis ) celatus , Pulmonaria officinalis , Salminus franciscanus , Thais chocolata and Zootoca vivipara . These loci were cross‐tested on the following species: Acanthina monodon , Alytes cisternasii , Alytes maurus , Alytes muletensis , Alytes obstetricans almogavarii , Alytes obstetricans boscai , Alytes obstetricans obstetricans , Alytes obstetricans pertinax , Cambarellus montezumae , Cambarellus zempoalensis , Chorus giganteus , Cobitis tetralineata , Glossina fuscipes fuscipes , Glossina pallidipes , Lysimachia japonica var. japonica , Lysimachia japonica var. minutissima , Orconectes virilis , Pacifastacus leniusculus , Procambarus clarkii , Salminus brasiliensis and Salminus hilarii .