Background and aims: Anticoagulation of the circuit used in continuous renal replacement therapy (CRRT) is an important technical aspect. Most studies evaluating the efficacy and safety of citrate use have been made in adult being having scant evidence available in pediatrics. Aims: The total hemofilter´s durability and haemofilter´s time in dialysis therapy were compared in hemofilters anticoagulated with heparin vs. regional citrate anticoagulation, within a Children´s population on CRRT in a pediatric center of Colombia. Methods: A total of 150 hemofilters (80 in the citrate group and 70 in the heparin group) and 3442 hours of therapy (2248 in the citrate group and 1194 in the heparin group) were included. The Hemofilters survival was estimated in two different situations. Initially, starting from the hemofilter placement until its retirement, caused by a clot presence or high trans membrane pressures (total durability). Subsequently, taking into account only the haemofilter´s time used in dialysis therapy (haemofilter´s time in therapy). This study was approved by the institutional ethics committee. Results: The total hemofilter´s durability, as well as the median haemofilter´s time in therapy, were higher in the citrate group (72 vs. 18 hours p< 0.0001; 30 vs. 12 hours p< 0.00001, respectively). The hemofilter´s coagulation risk was significantly increased when heparin was used, despite the hemodilter size and pump flow (HR=3.70; SE 0.82; CI 95%, 2.39–5.72, p<0.00001). Conclusions: Regional citrate anticoagulation is more effective than heparin systemic anticoagulation in terms of prolonging the duration of the haemofilter. This may be reflected in CRRT cost decrease in children.