Parkinson's disease is characterized by alterations in the gait pattern that may increase the risk of falls. Variations in the gait pattern cannot be objectively measured in clinical examination, so it is necessary to adapt devices to measure objectively, valid and replicable changes in gait patterns that are part of the evolution of the disease and / or pharmacotherapy. In an interdisciplinary effort, we developed the "e-Motion Capture System" software, which is able to calculate motor (cadence, stride and step length) and spatiotemporal (velocity and acceleration) parameters that affect quality of life in patients with Parkinson's disease. In this paper, we show results of the comparison between our e-Motion software and a benchmark reference, multiple-camera 3D motion capture system to track a gait pattern. This analysis was performed to compare the spatial locations of the ankles of a volunteer under indoor controlled conditions. Our results for the comparison between e-Motion and the 3D motion capture system show excellent agreement.