ABSTRACT Objective. The study was carried out in two farms, located in São Sebastião do Passé, Bahia, Brazil, during the three first months of lactation of 81 female buffaloes, to observe if stranger people at milk parlor could affect the milk production. Materials and methods. Four strangers were at the milking parlor during the milking time to observe the following parameters: Duration of Stay in the Milking Parlor; Milking Period; Rumination; Defecation; Urination Reactivity; Stress Level; Side Preference in the Milking Parlor ; Milk Yield, and Post Control Milk Yield. The females were categorized as 1=primiparous, 2=pluriparous, and 3=when information about calving number was not available. The data were analyzed using SAS Statistical Package. Results. During the presence of four strangers at the milking parlor, it was observed that the mean value for Duration of Stay in the Milking Parlor was 23 minutes (varying from 12 to 38 minutes). Average Milking Period was 8 minutes (varying from 4 to 13 minutes). Average Milk Yield during observation days (MY) varied from 2.30 kg to 14.70 kg, with an average value of 7.70±2.60 kg, while Mean Milk Yield after the observation days was 8.600±2.70 kg. Conclusions. As far as Post Control Milk Yield, since the animals presented increased milk yield in both farms on the days following the observations, it is possible to assert that the presence of strangers in the milk parlor during milk control reduced milk yield only during the day of observation, with no adverse effects on subsequent milk production.