La litiasis biliar constituye un problema importante en los sistemas de salud occidentales, muchos estudios han identificado diversos factores de riesgo entre ellos el aumento en la saturacion de colesterol, es por ello que la mayoria de los calculos pueden ser disueltos con sales biliares, pero esto constituye una terapia costosa y poco accesible. En el presente estudio fueron estudiados todos los calculos obtenidos de 90 colecistectomias realizadas en el Hospital Universitario del Caribe, y se analizaron mediante la espectrometria infrarroja. Se encontro colesterol en la mayoria de los calculos analizados, acido pantotenico solo se encontro en uno. La mayoria de las colecistectomia presentaban multiples calculos, los cuales con mayor frecuencia eran ovalados y de color cafe y tamanos variables que van desde 8 mm hasta mayores de 3 cms. Rev. cienc.biomed.2010:1(2): 180 - 184 PALABRAS CLAVES Calculos biliares. Composicion. Bilirrubina. Colesterol. SUMMARY The biliary lithiasis is a major problem in Western health systems; many studies have identified several risk factors including increased cholesterol saturation, which is why most of the gallstones can be dissolved with bile salts, but this constitutes a very expensive and not widely available therapy. In the present study, all the gallstones obtained from 90 cholecystectomies performed at the Hospital Universitario del Caribe were studied, and analyzed by infrared spectrometry. Cholesterol is found in most of the gallstones discussed, and pantothenic acid was only found in one. Most cholecystectomies had multiple gallstones, which were most often oval, brown and of various sizes, ranging from 0.8 to over 3 cm. KEYWORDS Gallstones. Composition. Bilirubin. Cholesterol.