Objective: to evaluate the implementation of tuberculosis infection control measures in administrative, environmental and respiratory protection equipment in a third level hospital in Bogota D.C. Methodology: a descriptive, cross-sectional study. Data were collected through a self-reporting instrument applied on 158 health care workers. The chief officer of the institutional program for tuberculosis control participated in a structured interview. In addition, an expert in occupational health and safety conducted an observational visit to the different workplaces in order to inquire on the implementation of tuberculosis infection control measures at the health facilities. Results: both, the chief officer of the institutional program for tuberculosis control and the 158 health care workers reported a lack of tuberculosis infection control measures at the administrative and environmental levels, as well as in the matters concerning respiratory protection equipment. This was confirmed by the expert. Conclusions: Certain working conditions coupled with poor implementation of tuberculosis infection control measures in health care institutions may favor the health care workers' occupational exposure to Mycobacterium Tuberculosis.