A new species of Penaincisalia from the central mountain range of Colombia is described and diagnosed. We present a brief discussion on the systematic placement of Penaincisalia cuiva Prieto & Rodríguez n. sp. within the genus. The species is apparently a member of the “browni group”, and we discuss its relationships with other members of this group. Adult specimens of both sexes and the genitalia of the male are illustrated and compared with P. magnifica, the most closely related species according to phenotypic appearance. A key to the known species of the “browni group” is provided.