Specimens of laminated bamboo Guadua angustifolia do not usually fail due to fiber breakage when submitted to shear stresses. The common failure mechanism in that case is slippage in the fiber-matrix interface, accompanied by degradation of the lignin matrix (parenchyma). In this study the shear strength of laminated bamboo Guadua angustifolia specimens was determined by tension tests reducing the cross section area. The perimeter of the slipped area was determined using digital image processing. Shear stresses were calculated taking into account the load and slipped area in specimens of two different groups, depending on the orientation of the laminated boards. It was found that the average value of the shear strength on pull out tests were 2.9 MPa, which is a mechanical property of the material that is useful, for example, in the analysis of the behavior of the joints, and analysis of the fracture process.