Introduction. Leptospirosis is the most disseminated zoonotic disease worldwide, and it causes serious economic loses in several animal species. There are few serological studies in Colombia applied to horses, so the reactivity of the main serovars is unknown. Objective. Determine the sanitary status of leptospira spp in apparently healthy horses and identify the main serovars. At the same time, we intend to relate seropositivity with the age, the age group and the sex of the animals. Materials and methods. A transversal study was performed in (n=94) horses from four towns located in the Guaviare and Meta provinces. As a diagnostic test, the microscopic agglutination (MAT) test was used before 10 serovars. Results. The seroprevalence in the subpopulations were L. Pomona, 41.5 %; L icterohaemorragiae, 40.4 %; L. grippothyposa, 24.5 %; L. javanica 23.4 %; L. canicola, 16 %; L. hardjoprajitno 10.6 %; L tarassovi, 7,4 %, L. hebdomadis, 7,4 %; L. wolffi, 2,1 % and L. Bratislava, 1,1 %. 23.4 % of the animals had no serological reactivity, and the response to one or more serovars was 76.6 %. Conclusion. The most prevalent serovars were Pomona and cterohaemorragaiae, and this can be a reflex of the specific epidemiological conditions of the towns the animals come from.