The aim of this study was to estimate emissions of greenhouse gases (GHG) generated by the agricultural activities carried out in the Metropolitan Area of the Aburrá Valley (AMVA), located in Medellin - Colombia. A TIER 1 approach of the methodology of the Intergovernmental Panel on Climate Change, IPCC was followed. Emissions of GHG from cropland, aggregate sources and non-CO2 emissions from land were estimated and analysis of the uncertainty of activity data and emission factors were made. The estimated total emission was 63.1 and 66 Gg CO2 eq for 2009 and 2011, respectively. The greatest contribution to greenhouse gases in agricultural production was the application of nitrogen to soils in the form of synthetic and organic fertilizers, which was associated with direct and indirect N2O emissions. The main sources of uncertainty were those derived from the activity data.