Apteronotus eschmeyeri n. sp. is described here from the Magdalena Basin in Colombia and distinguished from congeners by the following combination of features: blotchy coloration on body, clear band from chin to the beginning of dorsal mid-saggital electroreceptororgan, two clear bands surrounding the caudal peduncle in specimens up to 165 mm TL, mouth rictus passing the posterior margin of orbit. The present work demonstrates that the Trans-Andean apteronotids may be more diverse than previously supposed.