A new genus, Calima, and two new species, C. bremensis and C. valenciorum, of Hubbardiidae are here described,respectively from Bremen Forest, Filandia, Quindio department and Andinapolis, Trujillo, Valle del Cauca department,both localities located in the Colombian Andes. A comprehensive map of the South American species with a four-segmented female flagellum is presented, tables with characters of New World Hubbardiidae genera are provided. The relationships of the new genus within neotropical Hubbardiidae fauna is discussed.